Ọrịa ịba ọcha n'anya D IgM ELISA Kit

Ngwa a na-achọpụta nje ịba ọcha n'anya D IgM antibody (HDV-IgM) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, a na-ekpuchiri polystyrene microwell ibe ya na ọgwụ mgbochi na-eduga na protein immunoglobulin M mmadụ (mgbochi μ chain).Mgbe mbu gbakwunyere serum ma ọ bụ plasma ka a ga-enyocha, enwere ike weghara ọgwụ mgbochi IgM dị na ụdị ahụ, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ (gụnyere ọgwụ mgbochi IgG akọwapụtara) site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) antigens conjugated ga-emeghachi omume kpọmkwem na HDV IgM antibodies.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ebe ọnụnọ (mgbochi μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, mgbe ịsachara efere ahụ, a na-agbakwunye mkpụrụ TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume nke agba agba agba agba. mepụta ihe na-acha anụnụ anụnụ, tinye 50μl nke Kwụsị Ngwọta, wee gbanwee edo edo.Ọnụnọ nke nnabata nke mgbochi HDV-IgM na nlele ahụ bụ onye na-agụ microplate kpebiri.