Enterovirus 71 (EV71) IgM ELISA Kit

Enterovirus 71(EV71) Ihe osise IgM ELISA egosipụtara

Nkọwa dị mkpirikpi:

Enterovirus 71 IgM (EV71-IgM) ELISA kit bụ ihe nyocha immunosorbent jikọtara enzyme maka nchọpụta qualitative nke ọgwụ mgbochi klaasị IgM na Enterovirus 71 na ọbara mmadụ ma ọ bụ plasma.Ezubere iji ya na ụlọ nyocha ụlọ ọgwụ maka nyocha na njikwa nke ndị ọrịa metụtara ọrịa Enterovirus 71.

Mmadụ enterovirus 71 (EV71), onye kachasị ọhụrụ nke Enteroviridae, bụ ihe na-akpatakarị ọrịa aka, ụkwụ na ọnụ (HFMD) na mgbe ụfọdụ a na-ejikọta ya na ọrịa usoro nhụjuanya dị ukwuu.Ọrịa na-ebutekarị ọrịa na June na Julaị.Ọrịa nwere EV71 nwere ike ịbụ asymptomatic ma ọ bụ nwee ike ibute afọ ọsịsa na rashes.Ụmụaka nọ n'okpuru afọ ise nọ n'ihe ize ndụ dị elu nke ịmalite ọrịa siri ike karịa ndị okenye na ụmụaka ndị toro eto.

A na-ebunye EV71 site na mmadụ gaa na onye site na kọntaktị ozugbo yana site na mmetọ aka ma ọ bụ ihe nwere nsị nje.Ihe nzuzo imi na akpịrị, asọ mmiri ma ọ bụ mmiri sitere na ọnya nwekwara ike gbasaa nje a.

Enwere ike kewapụ ma chọpụta EV71 n'ime akpịrị na stool site na iji ule PCR.Achọpụtala nje virus RNA na mmiri vesicle akpụkpọ, ọbara na mmamịrị.Enwere ike nweta nchọpụta mbụ na nke dị mfe site na iji EV71 akọwapụtara serological assays, gụnyere ule akọwapụtara maka mgbochi IgM.

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Ụkpụrụ

Ngwa a na-achọpụta Enterovirus 71 IgM antibody (EV71-IgM) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, a na-ekpuchi polystyrene microwell ibe ya na mgbochi nje na-eduga na protein immunoglobulin M nke mmadụ (mgbochi μ chain) .Mgbe mbụ gbakwunyere serum ma ọ bụ plasma specimens ga-abụ. enyochala ya, enwere ike ijide ọgwụ mgbochi IgM dị na ụdị ahụ, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ (gụnyere ọgwụ mgbochi IgG akọwapụtara) site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) -conjugated antigens ga-emeghachi omume kpọmkwem na EV71 IgM ọgwụ nje.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ihu (mgbochi μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume onye mmepụta agba pụta. iji mepụta ihe na-acha anụnụ anụnụ, tinye 50μL nke Kwụsị Ngwọta, wee gbanwee edo edo.Onye na-agụ microplate kpebiri ọnụnọ nke nnabata nke EV71-IgM mgbochi n'ime nlele ahụ.

Njirimara ngwaahịa

Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	Ụzọ ijide
Asambodo	CE
Ụdị	Ọbara mmadụ / plasma
Nkọwapụta	48T/96T
Ọnọdụ nchekwa	2-8 ℃
Ndụ nchekwa	Ọnwa iri na abụọ