Epstein Barr nje VCA IgM ELISA Kit

Ngwa Epstein Barr VCA IgM ELISA Ihe onyonyo egosipụtara

Nkọwa dị mkpirikpi:

A na-eji ya maka nchọpụta qualitative nke ọgwụ mgbochi klaasị IgM na nje Epstein-barr capsid antigen na serum ma ọ bụ plasma mmadụ.Ezubere ya ka ejiri ya na ụlọ nyocha ụlọ ọgwụ maka nyocha na njikwa nke ndị ọrịa metụtara nje Epstein-barr.

Ọrịa EBV juru ebe niile, yana ihe karịrị 90% nke ndị bi n'ụwa bu nje virus ahụ, yana EBV bụ ọrịa na-efe efe na-efe efe, nke na-ebute ọrịa mbụ na ụmụ ọhụrụ na ndị nọ n'afọ iri na ụma, ọkachasị asymptomatic, na ndị nọ n'afọ iri na ụma na-ebute mononucleosis na-efe efe.Mgbe ọrịa mbụ gasịrị, EBV na-emekarị ka ọ dị na lymphocytes B nke mmadụ tozuru okè.N'okpuru ọnọdụ ụfọdụ, enwere ike ịmegharị nje virus ahụ latent, na-akpali mgbasawanye cell na ọdịiche dị iche iche, na n'ọnọdụ ụfọdụ, na-emecha ghọọ ọrịa ọjọọ dị ka lymphoma, na-enwe ọganihu na-adịghị mma, ya mere nchọpụta mmalite nke EBV ekwesịghị ileghara anya.

EBV bụ otu n'ime ihe na-ebute ọtụtụ ọrịa ọjọọ (dịka ọmụmaatụ carcinoma nasopharyngeal) na ọ na-ebutekarị mkpụrụ ndụ epithelial na B lymphocytes na oropharynx mmadụ.Nnwale EBV nwere ule antibody na antigen.Nnwale mgbochi EBV gụnyere ọgwụ mgbochi metụtara viral capsid antigen (VCA), mmalite antigen (EA), viral nuclear antigen (EBNA), na membrane antigen (MA), na a na-ejikarị ụlọ ọgwụ chọpụta EB-VCA-IgM na EB-VCA. -IgG.Nnwale EB-VCA-IgM na-achọpụta ọgwụ mgbochi ọrịa n'oge nnukwu oge nke onye ọrịa, yana nsonaazụ dị mma maka ihe a bụ mmalite mmalite, nke akọwapụtara na nke nwere mmetụta maka nyocha ụlọ ọgwụ.

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Ụkpụrụ

Ngwa a na-eji ụkpụrụ nke usoro na-apụtaghị ìhè achọpụta EBVCA IgM antibody serum ma ọ bụ plasma samples, polystyrene microwell strips na-ebu ụzọ kpuchie ya na ọgwụ mgbochi na-eduga na protein immunoglobulin M mmadụ (mgbochi μ chain) .Mgbe mbụ gbakwụnye serum ma ọ bụ plasma specimens ga-enyocha. , Enwere ike ijide ọgwụ mgbochi IgM dị na ụdị ahụ, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ (gụnyere ọgwụ mgbochi IgG kpọmkwem) site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) -conjugated antigens ga-emeghachi omume nanị na ọgwụ nje EBV IgM.N'ikpeazụ, etinyere mkpụrụ osisi TMB maka mmepe agba.Ọnụnọ nke nnabata (A uru) nke mgbochi EBVCA IgM na nlele ahụ bụ onye na-agụ microplate kpebiri.

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Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	Ụzọ ijide
Asambodo	CE
Ụdị	Ọbara mmadụ / plasma
Nkọwapụta	48T/96T
Ọnọdụ nchekwa	2-8 ℃
Ndụ nchekwa	Ọnwa iri na abụọ