Ịba ọcha n'anya E nje IgM ELISA Kit

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A na-eji Cassette Nnwale ịba ọcha n'anya E nje IgM nyocha nke ọma nke nje ịba ọcha n'anya nje IgM na ọbara mmadụ, plasma (EDTA, heparin, sodium citrate) ma ọ bụ ọbara dum (EDTA, heparin, sodium citrate).A ga-eji ule a mee ihe dị ka ihe enyemaka n'ịchọpụta ọrịa ịba ọcha n'anya nke nje virus Hepatitis E kpatara.

Nje nje ịba ọcha n'anya E (HEV) bụ nje RNA na-anaghị ekpuchi ya, otu eriri RNA na-ebutekarị site n'ụzọ fecal-onu, mmịnye ọbara na ikekwe nne-nwa ebu n'afọ.Ọrịa na HEV na-ebute nnukwu ọrịa ịba ọcha n'anya na-efe efe ma na-ebute nnukwu ọrịa imeju ma ọ bụ nke subclinical yiri ịba ọcha n'anya A. Ebe enwere nnukwu genotypes anọ nke HEV, enwere naanị otu serotype.

Ọrịa HEV n'ime mmadụ na-emepụta ọgwụ mgbochi IgM, IgA na IgG.HEV-IgM na HEV-IgA positivity bụ ihe ịrịba ama nke nnukwu ma ọ bụ na-adịbeghị anya ọrịa HEV.Ma mgbochi HEV-IgM na mgbochi HEV-IgA dị mma maka otu ma ọ bụ abụọ, ha na-egosi ọrịa HEV na nso nso a.Ọnụnọ nke ọrịa HEV na-adịbeghị anya, jikọtara ya na ọrụ imeju, nwere ike iji chọpụta ma ọrịa ahụ ọ dị nnukwu ma ọ bụ na-adịbeghị anya.Enwere ike iji ọnụnọ nke ọrịa HEV na imeju chọpụta ma ọrịa ahụ ọ bụ nnukwu ịba ọcha n'anya E ma ọ bụ mgbake site na nnukwu ịba ọcha n'anya E.

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Ụkpụrụ

Ngwa a na-achọpụta nje ịba ọcha n'anya E IgM antibody (HEV-IgM) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, a na-ekpuchi eriri polystyrene microwell na mgbochi nje na-eduga na protein immunoglobulin M mmadụ (mgbochi μ chain).Mgbe mbu gbakwunyere serum ma ọ bụ plasma ka a ga-enyocha, enwere ike weghara ọgwụ mgbochi IgM dị na ụdị ahụ, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ (gụnyere ọgwụ mgbochi IgG akọwapụtara) site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) -conjugated antigens ga-emeghachi omume kpọmkwem naanị na mgbochi HEV IgM.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.Na ọnụnọ nke (mgbochi μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume nke agba agba agba. mepụta ihe na-acha anụnụ anụnụ, tinye 50μl nke Kwụsị Ngwọta, wee gbanwee edo edo.Ọnụnọ nke nnabata nke ihe mgbochi HEV-IgM na nlele ahụ bụ onye na-agụ microplate kpebiri.

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Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	Ụzọ ijide
Asambodo	CE
Ụdị	Ọbara mmadụ / plasma
Nkọwapụta	48T/96T
Ọnọdụ nchekwa	2-8 ℃
Ndụ nchekwa	Ọnwa iri na abụọ