Enterovirus 71 (EV71) IgG ELISA Kit

Nkọwa dị mkpirikpi:

Human Enterovirus 71 IgG ELISA Kit bụ ihe nyocha immunosorbent jikọtara enzyme maka nchọpụta qualitative nke IgG-class antibodies na Enterovirus 71 na serum ma ọ bụ plasma mmadụ.Ezubere iji ya na ụlọ nyocha ụlọ ọgwụ maka nyocha na njikwa nke ndị ọrịa metụtara ọrịa Enterovirus 71.

Isi ihe ngosipụta nke ọrịa EV71 bụ ọrịa aka, ụkwụ na ọnụ.Ụfọdụ ụmụaka nwere ọrịa EV71 na-enwe pharyngitis herpetic, na n'ọnọdụ siri ike, nje encephalitis, nje encephalomyelitis, pulmonary edema na pulmonary hemorrhage.

Nchoputa a kapịrị ọnụ nke ọrịa EV71 na-adabere na nchọpụta EV71-RNA, EV71-IgM na EV71-IgG mgbochi n'ime ọbara, ma ọ bụ EV71-RNA na ụdị swab.

EV71-IgM bụ ihe dị mkpa na-egosi ọrịa mbụ ma ọ bụ na nso nso a ma na-akwado nchọpụta mmalite na ọgwụgwọ nke ọrịa EV71.EV71-IgG bụ ihe dị mkpa maka nchọpụta dị iche iche nke ọrịa na enwere ike iji ya mee nchọpụta ọrịa na nyocha nke ịgba ọgwụ mgbochi ọrịa.Enwere ike iji ya mee nchọpụta ọrịa na nyocha nke mmetụta ịgba ọgwụ mgbochi ọrịa.Mgbanwe nke antibody titers na ma gbakere na nnukwu ọbara nwekwara ike ikpebi ọrịa EV71, ma ọ bụrụ na titer nke serum antibody na-abawanye geometrically site na 4 n'ime oge enwetara ma e jiri ya tụnyere nnukwu oge, mgbe ahụ enwere ike ikpebi ya dị ka EV71 na-ebute ọrịa.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụkpụrụ

Ngwa a na-achọpụta ihe mgbochi Enterovirus 71 IgG (EV71-IgG) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, ihe mkpuchi microwell polystyrene ejiri ya na Enterovirus 71 antigen kpuchie ya.Mgbe mbụ gbakwụnye serum ma ọ bụ plasma ka a ga-enyocha, ọgwụ mgbochi ndị kwekọrọ na ya (EV71-IgG-Ab & ụfọdụ IgM-Ab) dị na ụdị ndị ọrịa na-ejikọta na antigens na oge siri ike, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) - mgbochi mmadụ IgG ga-emeghachi omume naanị na ọgwụ nje EV71 IgG.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ihu (EV71 Ag) - (EV71-IgG) - (mgbochi mmadụ IgG-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume nke agba agba. iji mepụta ihe na-acha anụnụ anụnụ, tinye 50μL nke Kwụsị Ngwọta, wee gbanwee edo edo.Onye na-agụ microplate kpebiri ọnụnọ nke nnabata nke EV71-IgG mgbochi n'ime nlele ahụ.

Njirimara ngwaahịa

Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ Enzyme jikọtara immunosorbent nyocha
Ụdị Usoro na-apụtaghị ìhè
Asambodo CE
Ụdị Ọbara mmadụ / plasma
Nkọwapụta 48T/96T
Ọnọdụ nchekwa 2-8 ℃
Ndụ nchekwa Ọnwa iri na abụọ

Ozi ịtụ

Aha ngwaahịa Mkpọ Ụdị
Enterovirus 71 (EV71) IgG ELISA Kit 48T/96T Ọbara mmadụ / plasma

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