Enterovirus 71 (EV71) IgG ELISA Kit
Ụkpụrụ
Ngwa a na-achọpụta ihe mgbochi Enterovirus 71 IgG (EV71-IgG) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, ihe mkpuchi microwell polystyrene ejiri ya na Enterovirus 71 antigen kpuchie ya.Mgbe mbụ gbakwụnye serum ma ọ bụ plasma ka a ga-enyocha, ọgwụ mgbochi ndị kwekọrọ na ya (EV71-IgG-Ab & ụfọdụ IgM-Ab) dị na ụdị ndị ọrịa na-ejikọta na antigens na oge siri ike, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) - mgbochi mmadụ IgG ga-emeghachi omume naanị na ọgwụ nje EV71 IgG.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ihu (EV71 Ag) - (EV71-IgG) - (mgbochi mmadụ IgG-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume nke agba agba. iji mepụta ihe na-acha anụnụ anụnụ, tinye 50μL nke Kwụsị Ngwọta, wee gbanwee edo edo.Onye na-agụ microplate kpebiri ọnụnọ nke nnabata nke EV71-IgG mgbochi n'ime nlele ahụ.
Njirimara ngwaahịa
Mmetụta dị elu, nkọwapụta na nkwụsi ike
Nkọwapụta ngwaahịa
Ụkpụrụ | Enzyme jikọtara immunosorbent nyocha |
Ụdị | Usoro na-apụtaghị ìhè |
Asambodo | CE |
Ụdị | Ọbara mmadụ / plasma |
Nkọwapụta | 48T/96T |
Ọnọdụ nchekwa | 2-8 ℃ |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ozi ịtụ
Aha ngwaahịa | Mkpọ | Ụdị |
Enterovirus 71 (EV71) IgG ELISA Kit | 48T/96T | Ọbara mmadụ / plasma |