Ngwakọta ule SARS-COV-2 (Elisa)

Ngwa SARS-CoV-2 Total Ab Test Kit (ELISA) dabere na nyocha nke immunoenzymatic maka mkpebi siri ike nke SARS-CoV-2 mgbochi n'ime ọbara mmadụ, plasma (EDTA, Heparin ma ọ bụ sodium citrate).A na-ekpuchi olulu mmiri micro efere dị ka akụkụ siri ike na protein recombinant SARS-CoV-2 recombinant Receptor Binding Domain.N'ime usoro nnabata nke mbụ kwekọrọ na ọgwụ mgbochi ụfọdụ (SARS-CoV-2-IgG-Ab & ụfọdụ IgM-Ab) dị na ụdị ndị ọrịa na-ejikọta na antigens n'oge siri ike.Na njedebe nke incubation unbound components na-asachapụ.Maka usoro nnabata nke abụọ SARS-CoV-2 recombinant recombinant Receptor Binding Domain protein conjugate (SARS-CoV-2 recombinant Receptor Binding Domain protein peroxidase conjugate) agbakwunyere nke jikọtara ya na nje SARS-CoV-2 (gụnyere IgG na IgM) na-akpata. nguzobe nke ahụkarị immunocomplexes.Ka emechara usoro ịsa ahụ nke abụọ iji wepụ ngafe conjugate, a na-agbakwunye TMB/Substrate (step3).Agba na-acha anụnụ anụnụ na-etolite na-agbanwe na-acha odo odo mgbe ịkwụsị mmeghachi omume na ngwọta nkwụsị.A na-ekpebi nnabata nke calibrators na ihe nlere site na iji ihe na-agụ efere ELISA.A na-enweta nsonaazụ maka nlele onye ọrịa site n'ịtụnyere uru mbelata.