Nje Virus Measles (MV) IgG ELISA Kit

Ngwa a na-achọpụta Measles virus IgG antibody (MV-IgG) na ọbara mmadụ ma ọ bụ ihe nlele plasma, a na-eji antigen nje Measles kpuchie polystyrene microwell ibe.Mgbe mbụ gbakwụnye serum ma ọ bụ plasma ka a ga-enyocha, ọgwụ mgbochi ndị kwekọrọ na ya (MV-IgG-Ab & ụfọdụ IgM-Ab) dị na ụdị ndị ọrịa na-ejikọta na antigens n'oge siri ike, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) - mgbochi mmadụ IgG ga-emeghachi omume naanị na ọgwụ mgbochi MV IgG.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ihu (MV Ag) - (MV-IgG) - (mgbochi mmadụ IgG-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume onye mmepụta agba. iji mepụta ihe na-acha anụnụ anụnụ, tinye 50μl nke Kwụsị Ngwọta, ma gbanwee edo edo. Ọnụnọ nke ntinye nke MV-IgG antibody na ihe nlele ahụ bụ onye na-agụ microplate kpebiri.