Mycoplasma Pneumonia IgM ELISA Kit

Ngwa Mycoplasma Pneumonia IgM ELISA egosipụtara

Nkọwa dị mkpirikpi:

Mycoplasma Pneumoniae IgM (MP-IgM) ELISA Kit bụ nyocha immunosorbent jikọrọ enzyme maka nchọpụta qualitative nke ọgwụ mgbochi klaasị IgM na Mycoplasma Pneumoniae na ọbara mmadụ ma ọ bụ plasma.Ezubere ya ka ejiri ya na ụlọ nyocha ụlọ ọgwụ maka nyocha na njikwa nke ndị ọrịa metụtara ọrịa Mycoplasma Pneumoniae.Mycoplasma pneumoniae (MP) bụ microorganism dị n'etiti nje na nje bacteria.Ọ bụ obere microorganism cellular prokaryotic nke nwere ike itolite ma mụbaa na mgbasa ozi na-adịghị ndụ.Na mgbakwunye na mgbaàmà, ọ nwekwara ike ịkpata ụkwara ume ọkụ, nnukwu ọgụ nke ọrịa na-egbochi akpa ume, ma mee ka ọtụtụ sistemu mebie akwara obi, ọbara, na akwara akwara.Enweghị nkọwa dị iche iche na ngosipụta ụlọ ọgwụ na ọ dịghị mma maka nyocha.Ya mere, ịmepụta usoro nyocha ngwa ngwa na ngwa ngwa dị oke mkpa na-eduzi ọgwụgwọ ụlọ ọgwụ.

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Ụkpụrụ

Ngwa a na-achọpụta Mycoplasma Pneumoniae IgM (MP-IgM) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, polystyrene microwell strips na-eji ọgwụ mgbochi na-eduga na protein immunoglobulin M mmadụ (ant-μ chain) .Mgbe mbụ gbakwụnye serum ma ọ bụ plasma specimens ga-enyocha. , Enwere ike ijide ọgwụ mgbochi IgM dị na ụdị ahụ, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ (gụnyere ọgwụ mgbochi IgG kpọmkwem) site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) antigens conjugated ga-emeghachi omume naanị na ọgwụ mgbochi MP IgM.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ebe ọnụnọ (mgbochi μ) - (MP-IgM) - (MP-Ag-HRP) immunocomplex, mgbe ịsachara efere ahụ, agbakwunyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume nke agba agba agba. iji mepụta ihe na-acha anụnụ anụnụ, tinye 50μl nke Kwụsị Ngwọta, wee gbanwee edo edo.Onye na-agụ microplate kpebiri ọnụnọ nke nnabata nke ọgwụ mgbochi MP-IgM na nlele ahụ.

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Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	Ụzọ ijide
Asambodo	NMPA
Ụdị	Ọbara mmadụ / plasma
Nkọwapụta	96T
Ọnọdụ nchekwa	2-8 ℃
Ndụ nchekwa	Ọnwa iri na abụọ