Ngwa Anti-SARS-CoV-2 na-egbochi mmadụ ime ihe mgbochi (ELISA)
Ụkpụrụ
Ngwa ahụ na-eji ụkpụrụ nke nyocha asọmpi iji chọpụta ọgwụ mgbochi mgbochi megide SARS-CoV-2 na serum ma ọ bụ ihe nlele plasma.Nke mbụ, ihe nlele a ga-anwale, njikwa dị mma na njikwa na-adịghị mma na-agwakọta ya na HRP-RBD ka ihe mgbochi mgbochi na-ejikọta na HRP-RBD, mgbe ahụ, a na-agbakwunye ngwakọta ahụ na efere nwude nke ejiri protein hACE2 kpuchie ya.A ga-ejide HRP-RBD n'imebi mgbochi mgbochi yana HRP-RBD ọ bụla na-ejikọta ya na mgbochi na-adịghị ekewapụ onwe ya na efere ahụ, ebe a na-ewepụ ihe mgbagwoju anya HRP-RBD na neutralizing antibody n'oge ịsa ahụ.Agbakwunyere ngwọta TMB iji mepụta agba.N'ikpeazụ, agbakwunyere ngwọta nkwụsị ma kwụsịchaa mmeghachi omume ahụ.Ọnụnọ ma ọ bụ enweghị Anti-SARS-CoV-2 antibody na-ewepụ ihe mgbochi na nlele ahụ ka onye na-agụ microplate na-achọpụta nnabata (A-uru).
Njirimara ngwaahịa
Mmetụta dị elu, nkọwapụta na nkwụsi ike
Nkọwapụta ngwaahịa
Ụkpụrụ | Enzyme jikọtara immunosorbent nyocha |
Ụdị | Usoro asọmpi |
Asambodo | CE |
Ụdị | Ọbara mmadụ / plasma |
Nkọwapụta | 96T |
Ọnọdụ nchekwa | 2-8 ℃ |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ozi ịtụ
Aha ngwaahịa | Mkpọ | Ụdị |
Ngwa Anti-SARS-CoV-2 na-egbochi mmadụ ime ihe mgbochi (ELISA) | 96T | Ọbara mmadụ / plasma |