Ngwa Anti-SARS-CoV-2 na-egbochi mmadụ ime ihe mgbochi (ELISA)

Nkọwa dị mkpirikpi:

Ngwaahịa a na-eji nyocha nke immunosorbent jikọtara enzyme iji chọpụta nke ọma ọgwụ mgbochi ọ bụla na serum na plasma nke nwere ike iwepu mmekọrịta RBD-ACE2, yana usoro a ejedebeghị site na ụdị ụdị mkpụrụ ndụ ihe nketa na ngalaba mgbochi mmadụ.Ọgwụ mgbochi na-anọpụ iche nwere ike igbochi viral spike glycoprotein receptor binding region (RBD) ka ya na cell receptor receptor angiotensin-converting enzyme-2 (ACE2) na-emekọrịta ihe.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụkpụrụ

Ngwa ahụ na-eji ụkpụrụ nke nyocha asọmpi iji chọpụta ọgwụ mgbochi mgbochi megide SARS-CoV-2 na serum ma ọ bụ ihe nlele plasma.Nke mbụ, ihe nlele a ga-anwale, njikwa dị mma na njikwa na-adịghị mma na-agwakọta ya na HRP-RBD ka ihe mgbochi mgbochi na-ejikọta na HRP-RBD, mgbe ahụ, a na-agbakwunye ngwakọta ahụ na efere nwude nke ejiri protein hACE2 kpuchie ya.A ga-ejide HRP-RBD n'imebi mgbochi mgbochi yana HRP-RBD ọ bụla na-ejikọta ya na mgbochi na-adịghị ekewapụ onwe ya na efere ahụ, ebe a na-ewepụ ihe mgbagwoju anya HRP-RBD na neutralizing antibody n'oge ịsa ahụ.Agbakwunyere ngwọta TMB iji mepụta agba.N'ikpeazụ, agbakwunyere ngwọta nkwụsị ma kwụsịchaa mmeghachi omume ahụ.Ọnụnọ ma ọ bụ enweghị Anti-SARS-CoV-2 antibody na-ewepụ ihe mgbochi na nlele ahụ ka onye na-agụ microplate na-achọpụta nnabata (A-uru).

Njirimara ngwaahịa

Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ Enzyme jikọtara immunosorbent nyocha
Ụdị Usoro asọmpi
Asambodo CE
Ụdị Ọbara mmadụ / plasma
Nkọwapụta 96T
Ọnọdụ nchekwa 2-8 ℃
Ndụ nchekwa Ọnwa iri na abụọ

Ozi ịtụ

Aha ngwaahịa Mkpọ Ụdị
Ngwa Anti-SARS-CoV-2 na-egbochi mmadụ ime ihe mgbochi (ELISA) 96T Ọbara mmadụ / plasma

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