Toxoplasma IgG ELISA Kit

Ngwa a na-achọpụta Toxoplasma lgG antibody (TOX-lgG) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, eriri polystyrene microwell ejiri Toxoplasma antigen kpuchie ya.Mgbe mbụ gbakwụnye serum ma ọ bụ plasma ka a ga-enyocha, ọgwụ mgbochi ndị kwekọrọ na ya (TOX-lgG-Ab & ụfọdụ lgM-Ab) dị na ụdị ndị ọrịa na-ejikọta na antigens n'oge siri ike, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) -conjugated mgbochi mmadụ lgG ga-emeghachi omume naanị na ọgwụ mgbochi TOX lgG.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta , a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ihu (TOX Ag) - (TOX-lgG) - (mgbochi mmadụ lgG-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume onye mmepụta agba. iji mepụta ihe na-acha anụnụ anụnụ, tinye 50 µ I nke Stop Solution, wee tụgharịa edo edo.Ọnụnọ nke nnabata nke mgbochi TOX-lgG dị na nlele ahụ bụ onye na-agụ microplate kpebiri.