Nnwale nchọpụta TB-IGRA

Nkọwa dị mkpirikpi:

Nnwale nchọpụta TB-IGRA, nke a na-akpọkwa Interferon Gamma Release Assay, bụ ELISA maka nchọpụta ọnụọgụ nke Interferon Gamma (IFN-γ) nke na-anabata mkpali in vitro site na Mycobacterium Tuberculosis antigens na nlele ọbara mmadụ.TB-IGRA na-enyocha mmeghachi omume mgbochi ọrịa nke ụkwara nta Mycobacterium.E bu n'obi were nyocha a ka ọ bụrụ enyemaka n'ịchọpụta ọrịa TB gụnyere ma ọrịa ụkwara nta latent na ọrịa ụkwara nta.

Nzaghachi nke mgbochi ọrịa ụkwara nta nke Mycobacterium kpatara na-abụkarị nzaghachi cellular.Mgbe ọrịa ụkwara nta nke Mycobacterium butere, ahụ na-emepụta mkpụrụ ndụ T kpọmkwem ebe nchekwa nke na-ekesa n'ime ọbara dị n'akụkụ.Nleta nke IFN-γ megide ụkwara nta Mycobacterium egosila na ọ bụ usoro dị irè maka ịchọpụta ọrịa TB (ma latent ma na-arụ ọrụ), nke a na-akpọ IFN-γ in vitro release assay (IGRA).Ihe dị iche na ule tuberculin (TST) bụ na IGRA na-ahọrọ antigens kpọmkwem nke dị naanị na ụkwara nta Mycobacterium mana adịghị na BCG na ọtụtụ mycobacteria na-abụghị ụkwara nta.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụkpụrụ

Ngwa ahụ na-anakwere nyocha ntọhapụ interferon-γ maka ụkwara nta Mycobacterium (TB-IGRA) iji tụọ ike nke nzaghachi mgbochi cellular nke Mycobacterium ụkwara nta na-ahụ maka antigen kpọmkwem.
Nyocha nyocha nke immunosorbent jikọtara Enzyme yana ụkpụrụ Sanwichi mgbochi abụọ.
• Ejikwa ọgwụ mgbochi IFN-γ kpuchie microplates ndị ahụ.
• A na-agbakwunye ihe nlere anya nke a ga-anwale n'ime olulu mmiri microplate mkpuchi antibody, wee tinye horseradish peroxidase (HRP) mgbochi mgbochi IFN-γ n'ime olulu mmiri dị iche iche.
• IFN-γ, ọ bụrụ na ọ dị, ga-etolite mgbagwoju anya Sanwichi nwere mgbochi IFN-γ na mgbochi HRP-conjugated IFN-γ.
• A ga-emepe agba agba mgbe ị gbakwunyere ihe ngwọta nke mkpụrụ, ọ ga-agbanwe mgbe ọ gbakwụnyere nkwụsị nkwụsị.A na-eji onye na-agụ ELISA tụọ nnabata ahụ (OD).
• Ntụle IFN-γ dị na nlele ahụ jikọtara ya na OD kpebisiri ike.

Njirimara ngwaahịa

Nchọpụta ELISA dị irè maka ọrịa TB na-adịghị ala na nke na-arụ ọrụ

Enweghị nnyonye anya sitere na ọgwụ mgbochi BCG

Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	Usoro Sanwichi
Asambodo	CE, NMPA
Ụdị	Ọbara dum
Nkọwapụta	48T (chọpụta 11 samples);96T (chọpụta 27 samples)
Ọnọdụ nchekwa	2-8 ℃
Ndụ nchekwa	Ọnwa iri na abụọ