Rubella nje IgM ELISA Kit

Ngwa a na-achọpụta nje Rubella IgM antibody (RV-IgM) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, a na-ekpuchi eriri polystyrene microwell na mgbochi nje na-eduga na protein immunoglobulin M mmadụ (agbụ mgbochi µ).Mgbe mbu gbakwunyere serum ma ọ bụ plasma ka a ga-enyocha, enwere ike weghara ọgwụ mgbochi IgM dị na ụdị ahụ, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ (gụnyere ọgwụ mgbochi IgG akọwapụtara) site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) antigens conjugated ga-emeghachi omume naanị na ọgwụ mgbochi RV IgM.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ebe ọnụnọ (mgbochi) - (RV-lgM) - (RV-lgM) - (RV Ag-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume nke onye mmepụta agba na mepụta ihe na-acha anụnụ anụnụ, tinye 50 µI nke Stop Solution, wee tụgharịa edo edo.Onye na-agụ microplate kpebiri ọnụnọ nke nnabata nke mgbochi RV-IgM na nlele ahụ.