Nchọpụta nke HFRS - Ahụ ọkụ Hemorrhagic na Renal Syndrome

ndabere

Nje Virus Hantaan (HV) bụ isi ihe na-akpata ahụ ọkụ Hemorrhagic na Renal Syndrome (HFRS). HFRS bụ nnukwu ọrịa na-efe efe nke zoonotic na-ekesa n'ụwa niile nke ahụ ọkụ, ọbara ọgbụgba, na nkwarụ gbasara akụrụ. Ọrịa ahụ nwere nnukwu mmalite, ọganihu ngwa ngwa, yana ọnụ ọgụgụ dị elu nke na-egbu egbu, na-etinye nnukwu ihe egwu na ahụike ọha. Rodents (dị ka Apodemus agrarius na Rattus norvegicus) bụ isi mmiri na vectors nke HV. Mbufe mmadụ na-ebute bụ isi site na aerosolized excreta (mmamịrị, feces, asu), kọntaktị kpọmkwem, ma ọ bụ vector taa. HFRS nwere ike ime n'ime afọ niile, ndị mmadụ n'ozuzu nwere ike ime ya. Dị ka ọnụ ọgụgụ WHO si kwuo, mba 32 n'ụwa nile akọpụtala ọrịa HV, na-enwekarị nnukwu ebe na East Asia, Europe, na Balkans.

Ihe nrịbama Antibody Biputere ọrịa HV

Na-esochi ọrịa HV, usoro ahụ ji alụso ọrịa ọgụ na-emepụta ọgwụ mgbochi ọrịa, ọkachasị HV-IgM na HV-IgG.

● Ọgwụ mgbochi HV-IgM: Na-eje ozi dị ka ihe nrịbama serological nke ọrịa mmalite, na-apụtakarị n'ime ụbọchị mgbaàmà mmalite, ma dị oke mkpa maka nyocha nke nnukwu oge.

● Ọgwụ nje HV-IgG: Na-apụta ma emechaa nwee ike ịdịgide ndụ, na-egosi ọrịa gara aga ma ọ bụ ahụ efe efe. Mmụba okpukpu anọ ma ọ bụ karịa na HV-IgG antibody titer n'etiti nnukwu na convalescent serum sample bụkwa nchoputa maka nnukwu ọrịa.

Ụzọ nchọpụta HV nkịtị

Ụzọ ụlọ nyocha dị ugbu a maka nchọpụta HV gụnyere iche iche nje, PCR, ELISA serological, na immunoassay gold colloidal.

● Omenala nje virus na PCR na-enye nkọwa dị elu mana ọ na-ewe oge, na-achọ teknụzụ, ma na-achọ ụlọ nyocha dị elu, na-egbochi iji ha eme ihe.

● Micro-immunofluorescence (MIF) na-enye ezi izi ezi mana ọ chọrọ microscope fluorescence na nkọwa ndị ọkachamara, na-amachibido ngwa ngwa.

● A na-anabata ELISA na colloidal gold assays na ntọala ụlọ ọgwụ n'ihi ịdị mfe ha, ọsọ ọsọ, nnukwu uche na nkọwapụta, yana ịdị mfe nke nchịkọta sample (serum / plasma).

Arụmọrụ ngwaahịa

Atụmatụ nyocha Beier Bio HV-IgM/IgG (ELISA).

● Ụdị ihe atụ: Serum, plasma

● Ihe nlere anya: Ma IgM na IgG assays na-eji nlele mbụ nke ọma na 1: 11 dilution (100µl sample diluent + 10µl sample), na-eme ka usoro ọrụ na-arụ ọrụ na ibelata ọrụ ndị ọrụ.

● Reagent dị njikere iji: Reagents niile adịla njikere ma ewezuga ihe nchekwa ịsacha (20× gbadoro). Agba agba maka njirimara dị mfe

● Usoro ntinye: 30 min / 30 min / 15 min; zuru oke akpaaka

● Ogologo Ogologo nchọpụta: 450 nm nwere ntụaka 630 nm

● Ihe mkpuchi: olulu mmiri 96 ma ọ bụ 48 nwere ike imebi, nke ọ bụla nwere koodu ngwaahịa e biri ebi maka ịchọta na ịdị mma.

Atụmatụ nyocha Beier Bio HV-IgM/IgG (Colloidal Gold)

● Ụdị Nlereanya: Ọbara

● Oge Nchọpụta: Nsonaazụ n'ime nkeji iri na ise; enweghị akụrụngwa ọzọ achọrọ; dị mma maka nyocha ngwa ngwa na ụlọ ọgwụ, mberede, na ntọala ndị ọrịa gbasasịrị

● Usoro: Tinye 10µl sample na kaadị ule nke ọma site na iji dropper; kọwapụta nsonaazụ n'ime nkeji 15-20

Ịrụ ụlọ ọgwụ nke HV-IgM (ELISA), HV-IgG (ELISA), na HV-IgM/IgG (Colloidal Gold)