Human Cytomegalovirus IgM ELISA Kit

Ngwa a na-achọpụta ihe mgbochi mmadụ cytomegalovirus IgM (HCMV-IgM) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, a na-ekpuchiri polystyrene microwell ibe ya na ọgwụ mgbochi na-eduga na protein immunoglobulin M mmadụ (mgbochi mgbochi µ yinye).Mgbe mbu gbakwunyere serum ma ọ bụ plasma ka a ga-enyocha, enwere ike weghara ọgwụ mgbochi IgM dị na ụdị ahụ, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ (gụnyere ọgwụ mgbochi IgG akọwapụtara) site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) -conjugated antigens ga-emeghachi omume kpọmkwem na HCMV IgM ọgwụ nje.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ebe ọnụnọ (mgbochi) -(HCMV-lgM) - (HCMV Ag-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume nke agba agba agba. mepụta ihe na-acha anụnụ anụnụ, tinye 50 µI nke Stop Solution, wee tụgharịa edo edo.Ọnụnọ nke nnabata nke mgbochi HCMV-IgM n'ime nlele ahụ bụ onye na-agụ microplate kpebiri.