Herpes Simplex nje IgM ELISA Kit

Ngwa a na-achọpụta Herpes Simplex Virus I lgM antibody (HSV1-lgM) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, a na-ekpuchiri eriri polystyrene microwell na mgbochi nje na-eduga na protein immunoglobulin M mmadụ (agbụ mgbochi µ).Mgbe mbu gbakwunyere serum ma ọ bụ plasma ka a ga-enyocha, enwere ike weghara ọgwụ mgbochi lgM dị na ụdị ahụ, a ga-ewepụ ihe ndị ọzọ na-enweghị njikọ (gụnyere ọgwụ mgbochi lgG akọwapụtara) site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) -conjugated antigens ga-emeghachi omume nanị na HSV1 lgM ọgwụ nje.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ihu (anti-µ) - (HSV1-lgM) - (HSV1 Ag-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume onye mmepụta agba na mepụta ihe na-acha anụnụ anụnụ, tinye 50 µ I nke Stop Solution, wee tụgharịa edo edo.Onye na-agụ microplate kpebiri ọnụnọ nke nnabata nke HSV1-lgM mgbochi n'ime nlele ahụ.