Herpes Simplex II IgM ELISA Kit

Ngwa a na-achọpụta nje virus Herpes Simplex II IgM antibody (HSV2-IgM) n'ime ọbara mmadụ ma ọ bụ ihe nlele plasma, a na-ekpuchiri polystyrene microwell ibe ya na ọgwụ mgbochi na-eduga na protein immunoglobulin M mmadụ (nti-µ yinye).Mgbe mbu gbakwunyere serum ma ọ bụ plasma ka a ga-enyocha, enwere ike weghara ọgwụ mgbochi IgM dị na ụdị ahụ, a ga-ewepụkwa ihe ndị ọzọ na-enweghị njikọ (gụnyere ọgwụ mgbochi IgG akọwapụtara) site na ịsacha.Na nzọụkwụ nke abụọ, HRP (horseradish peroxidase) antigens conjugated ga-emeghachi omume nanị na HSV2 IgM ọgwụ nje.Mgbe ịsachara iji wepụ HRP-conjugate na-adịghị ejikọta, a na-agbakwunye ngwọta chromogen n'ime olulu mmiri.N'ihu (anti-µ) - (HSV2-lgM) (HSV2-lgM) (HSV2 Ag-HRP) immunocomplex, mgbe ịsachara efere ahụ, etinyere mkpụrụ osisi TMB maka mmepe agba, na HRP jikọtara ya na mgbagwoju anya na-eme ka mmeghachi omume onye mmepụta agba na mepụta ihe na-acha anụnụ anụnụ, tinye 50 µ I nke Stop Solution, wee tụgharịa edo edo.Onye na-agụ microplate kpebiri ọnụnọ nke nnabata nke HSV2-IgM mgbochi n'ime nlele ahụ.