Mgbochi Trophoblast Cell Membrane (TA) Ngwa mgbochi ELISA

Mpempe mkpụrụ ndụ mgbochi-Trophoblast (TA) Ihe mgbochi ELISA egosipụtara

Nkọwa dị mkpirikpi:

A na-eji ngwaahịa a maka nchọpụta qualitative in vitro nke anti-trophoblast cell membrane antibodies na ọbara mmadụ. Mkpụrụ ndụ trophoblast bụ akụkụ dị mkpa nke placenta, na-arụ ọrụ dị oke egwu n'ịkụnye embrayo n'oge, nhazi placental, na ịnọgide na-enwe ndidi nke nne na nwa ebu n'afọ.

Anti-trophoblast cell membrane antibodies bụ autoantibodies na-elekwasị anya antigens n'elu nke trophoblast cell. Mgbe ọgwụ mgbochi ndị a pụtara n'ime ahụ, ha nwere ike ịwakpo mkpụrụ ndụ trophoblast, mebie nhazi na ọrụ ha, na-egbochi ntinye nke embrayo nkịtị, ma mebie nguzozi nke mgbochi n'etiti nne na nwa ebu n'afọ. Nke a nwere ike ibute ọdịda nkunye, ịtụrụ ime n'oge, ma ọ bụ nsogbu ịmụ nwa, na-aghọ ihe nwere ike ịkpata infertility autoimmune.

N'ụlọ ọgwụ, nchọpụta a na-adabara dị ka ihe inyeaka diagnostic ngwá ọrụ maka autoimmune infertility. Ọ na-enyere aka ịchọpụta ma mmebi ahụ na-alụso ọrịa ọgụ na mkpụrụ ndụ trophoblast na-etinye aka na pathogenesis nke infertility, na-enye ozi ntụaka dị mkpa maka ndị dọkịta iji dokwuo anya ihe kpatara enweghị ọmụmụ na ịmepụta usoro ọgwụgwọ kwesịrị ekwesị.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụkpụrụ

Ngwa a na-achọpụta ọgwụ mgbochi mkpụrụ ndụ trophoblast cell (TA-Ab) n'ime nlele ọbara mmadụ dabere na usoro na-apụtaghị ìhè, yana membranes cell trophoblast sachara nke ejiri dị ka antigen mkpuchi.

Usoro nnwale ahụ na-amalite site n'ịgbakwunye ihe nlele serum na olulu mmiri mmeghachi omume nke ejiri antigen kpuchie ya, na-esote ya. Ọ bụrụ na TA-Ab dị na nlele ahụ, ọ ga-ejikọta kpọmkwem na antigens cell membrane trophoblast mkpuchi na olulu mmiri, na-emepụta ogige antigen-antibody kwụsiri ike.

Mgbe ewepụchara ihe ndị a na-ekekọtaghị site na ịsa ahụ iji hụ na achọpụtara izi ezi, a na-agbakwunye conjugates enzyme n'olulu mmiri. Mgbakwunye nke abụọ na-enye ohere njikọ enzyme ndị a ijikọ na ogige antigen-antibody dị ugbu a. Mgbe ewepụtara ihe ngwọta nke TMB, enzyme dị na mgbagwoju anya na-eme ka mmeghachi omume na TMB pụta, na-emepụta mgbanwe agba a na-ahụ anya. N'ikpeazụ, onye na-agụ microplate na-atụle nnabata (A value), nke a na-eji achọpụta ọkwa TA-Ab na nlele ahụ.

Njirimara ngwaahịa

Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	IndirectUsoro
Asambodo	NMPA
Ụdị	Ọbara mmadụ / plasma
Nkọwapụta	48T /96T
Ọnọdụ nchekwa	2-8℃
Ndụ nchekwa	12ọnwa