Ihe mgbochi Ovarian (AO) ELISA Kit

Ihe mgbochi Ovarian (AO) Ihe mgbochi ELISA egosipụtara

Nkọwa dị mkpirikpi:

Ovary nwere akwa, zona pellucida, mkpụrụ ndụ granulosa, wdg, na ọkwa mmepe dị iche iche. Akụkụ nke ọ bụla nwere ike ịkpalite ọgwụ mgbochi-ovarian (AoAb) n'ihi nkwupụta antigen na-adịghị mma. Ntufu nke antigen nke ovarian kpatara site na mmerụ ahụ ovarian, ọrịa, ma ọ bụ mbufụt nwere ike ịkpata AoAb n'ime ndị mmadụ na-enweghị ọrụ mgbochi. AoAb na-emebikwa ovary ma mebie ọrụ uterine na placental, na-ebute enweghị ọmụmụ na ime ọpụpụ.

Achọpụtara AoAb na ndị ọrịa nwere ọdịda ovarian akaghi aka (POF) na amenorrhea mmalite, jikọtara ya na mmeghachi omume autoimmune. AoAb na mbụ na-ebelata ọmụmụ ma mechaa bute ọdịda ovarian. Ndị ọrịa na-amụghị nwa nwere ezigbo AoAb mana ọ nweghị POF nwere ike chere ihe egwu POF dị elu n'ọdịnihu, na-achọ nyocha nchekwa ovarian.

AoAb positivity dị elu na ndị ọrịa na-amụghị nwa na ime ime, na-egosi mmekọrịta chiri anya. Nnyocha na-egosi na AoAb na-ebute enweghị ọmụmụ karịa ime ime. Nnyocha e mere n'oge na-adịbeghị anya na-achọpụta AoAb n'ọtụtụ ndị ọrịa PCOS, na-atụ aro mbufụt nke ovarian na-ebute ọrịa na cytokines na-adịghị mma nwere ike ime ka PCOS na-amụbaghị ọmụmụ, nke chọrọ ọmụmụ ihe ọzọ.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụkpụrụ

Ngwa a na-achọpụta ọgwụ mgbochi-ovarian (IgG) n'ime nlele ọbara mmadụ dabere na usoro na-apụtaghị ìhè, yana antigens akpụkpọ anụ ovarian sachara nke ejiri maka mkpuchi mkpuchi microwells.

Usoro nnwale ahụ na-amalite site n'ịgbakwunye ihe nlele serum na olulu mmiri mmeghachi omume antigen precoated maka incubation. Ọ bụrụ na ọgwụ mgbochi-ovarian dị na nlele ahụ, ha ga-ejikọta kpọmkwem na antigens ovarian membrane antigens nke ekpuchiri mbụ na microwells, na-etolite ogige antigen-antibody kwụsiri ike. A na-ewepụsị ihe ndị na-ekekọtaghị ọnụ iji hụ na achọpụtara ya ziri ezi.

Na-esote, a na-agbakwunye horseradish peroxidase (HRP) -mgbochi mgbochi ụmụ mmadụ IgG n'olulu mmiri. Mgbe nnabata nke abụọ gasịrị, ọgwụ mgbochi ndị a na-akpọ enzyme na-ejikọta kpọmkwem na mgbochi-ovarian antibodies na ogige antigen-antibody dị ugbu a, na-eme ka "antigen-antibody-enzyme label" zuru oke nke na-alụso ọrịa ọgụ.

N'ikpeazụ, a na-agbakwunye ihe ngwọta TMB. HRP dị na mgbagwoju anya na-ebute mmeghachi omume kemịkalụ na TMB, na-emepụta mgbanwe agba a na-ahụ anya. A na-atụ ihe nnabata (A uru) nke ngwọta mmeghachi omume site na iji onye na-agụ microplate, na ọnụnọ ma ọ bụ enweghị mgbochi mgbochi ovarian na nlele ahụ na-ekpebi dabere na nsonaazụ nnabata.

Njirimara ngwaahịa

Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	IndirectUsoro
Asambodo	NMPA
Ụdị	Ọbara mmadụ / plasma
Nkọwapụta	48T /96T
Ọnọdụ nchekwa	2-8℃
Ndụ nchekwa	12ọnwa