Ngwa mgbochi insulin (INS) mgbochi ELISA
Ụkpụrụ
Ngwa a na-achọpụta ọgwụ mgbochi insulin (IgG) na nyocha ọbara mmadụ dabere na usoro na-apụtaghị ìhè, yana insulin mmadụ recombinant sachara nke ejiri dị ka antigen mkpuchi.
Usoro nnwale ahụ na-amalite site n'ịgbakwunye ihe nlele serum na olulu mmiri mmeghachi omume nke ejiri antigen kpuchie ya, na-esote ya. Ọ bụrụ na ọgwụ mgbochi insulin dị na nlele ahụ, ha ga-ejikọ kpọmkwem na insulin mmadụ recombinant mkpuchi n'olulu mmiri, na-etolite ogige antigen-antibody kwụsiri ike.
Mgbe ịsachara iji wepụ ihe ndị na-adịghị ejikọta ma zere nnyonye anya, a na-agbakwunye conjugates enzyme na olulu mmiri. Nzọụkwụ nnabata nke abụọ na-enye ohere njikọ enzyme ndị a ijikọ kpọmkwem na ogige antigen-antibody dị ugbu a. Mgbe ewebata ihe ngwọta nke TMB, mmeghachi omume agba na-apụta n'okpuru mmetụta catalytic nke enzyme na mgbagwoju anya. N'ikpeazụ, a na-eji onye na-agụ microplate na-atụ ihe ntinye aka (A value), nke na-enyere aka ikpebi ọnụnọ nke mgbochi insulin n'ime ihe nlele ahụ.
Njirimara ngwaahịa
Mmetụta dị elu, nkọwapụta na nkwụsi ike
Nkọwapụta ngwaahịa
| Ụkpụrụ | Enzyme jikọtara immunosorbent nyocha |
| Ụdị | IndirectUsoro |
| Asambodo | NMPA |
| Ụdị | Ọbara mmadụ / plasma |
| Nkọwapụta | 48T /96T |
| Ọnọdụ nchekwa | 2-8℃ |
| Ndụ nchekwa | 12ọnwa |
Ozi ịtụ
| Aha ngwaahịa | Mkpọ | Ụdị |
| mgbochiInsulin(INS) Ngwa mgbochi ELISA | 48T/96T | Ọbara mmadụ / plasma |







