Anti-Human Chorionic Gonadotropin (HCG) Ngwa mgbochi ELISA

Nkọwa dị mkpirikpi:

Ezubere ngwa a maka nchọpụta qualitative in vitro nke mgbochi chorionic gonadotropin mgbochi mmadụ (HCG-Ab) n'ime ọbara mmadụ.

HCG-Ab bụ autoantibody na ọkwa n'etiti isi ihe na-akpata infertility immunological. Human chorionic gonadotropin (HCG), hormone nke ime ime kpọmkwem nke syncytiotrophoblasts zoro ezo, bụ isi na-arụ ọrụ iji kwalite mmepe nke corpus luteum nke afọ ime na nzuzo nke hormones steroid. Ọ na-arụ ọrụ dị oke mkpa n'ịkwado afọ ime n'oge na igbochi njụ nke nne na nwa ebu n'afọ, na-eje ozi dị ka isi hormone maka ịkwado ime nwa ebu n'afọ.

A na-emepụta HCG-Ab nke abụọ mgbe ime ọpụpụ ma ọ bụ injections HCG gasịrị. Ihe dị ka 40% nke ndị mmadụ nwere akụkọ ntolite ime ọpụpụ na-anwale ihe dị mma maka HCG-Ab. Mgbe HCG-Ab na-ejikọta na HCG, ọ na-egbochi saịtị HCG na-arụ ọrụ ma na-egbochi ọrụ physiological ya, na-eme ka afọ ime ghara ịdịgide ma dị mfe na-eduga n'ọdịdị ime ma ọ bụ ugboro ugboro, nke n'aka nke ya na-ebute enweghị ọmụmụ. A na-akwado mmetụta ya na-akpata enweghị ọmụmụ site na ihe akaebe dị ka ihe isi ike dị na ịtụrụ ime mgbe ịgbanye HCG na mmetụta mgbochi mgbochi nke ọgwụ mgbochi HCG.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụkpụrụ

Ngwa a na-achọpụta ọgwụ mgbochi chorionic gonadotropin nke mmadụ na nyocha ọbara mmadụ dabere na usoro na-apụtaghị ìhè, yana antigens chorionic gonadotropin mmadụ sachara nke ejiri maka mkpuchi mkpuchi microwells.

Usoro nnwale ahụ na-amalite site n'ịgbakwunye ihe nlele serum na olulu mmiri mmeghachi omume antigen precoated maka incubation. Ọ bụrụ na mgbochi mmadụ chorionic gonadotropin mgbochi mmadụ dị na nlele ahụ, ha ga-ejikọta kpọmkwem na antigens kpuchiri ya na microwells, na-etolite ogige antigen-antibody kwụsiri ike.

Na-esote, a na-agbakwunye enzyme conjugates. Mgbe nnabata nke abụọ gasịrị, njikọ enzyme ndị a na-ejikọta na ogige antigen-antibody dị ugbu a. Mgbe etinyere mkpụrụ osisi TMB, mmeghachi omume agba na-apụta n'okpuru catalysis nke enzyme. N'ikpeazụ, onye na-agụ microplate na-atụ ihe ntinye (A value), nke a na-eji achọpụta ọnụnọ nke mgbochi chorionic gonadotropin mgbochi mmadụ na nlele.

Njirimara ngwaahịa

Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	IndirectUsoro
Asambodo	NMPA
Ụdị	Ọbara mmadụ / plasma
Nkọwapụta	48T /96T
Ọnọdụ nchekwa	2-8℃
Ndụ nchekwa	12ọnwa