Anti-Endometrial (EM) Ngwa ELISA Antibody

Anti-Endometrial (EM) Ngwa mgbochi ELISA egosipụtara

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative in vitro anti-endometrial antibodies (EmAb) na serum mmadụ.

EmAb bụ autoantibody na-elekwasị anya endometrium, na-ebute nzaghachi mgbochi. Ọ bụ ihe nrịbama mgbochi maka endometriosis yana jikọtara ime ọpụpụ nwanyị na enweghị ọmụmụ. Akụkọ na-egosi 37%-50% nke ndị ọrịa na-amụghị nwa, ime ọpụpụ ma ọ bụ endometriosis bụ EmAb-positive; ọnụ ọgụgụ ahụ ruru 24%-61% n'ime ụmụ nwanyị mgbe imechara ime.

EmAb na-ejikọta ya na antigens endometrial, na-emebi endometrium site na mmeju mmeju yana iwere mkpụrụ ndụ na-alụso ọrịa ọgụ, na-emebi ntinye embrayo na ịkpata ime ọpụpụ. Ọ na-ebikọkarị na endometriosis, yana ọnụọgụ nchọpụta nke 70%-80% na ndị ọrịa dị otú ahụ. Ngwa a na-enyere aka ịchọpụta endometriosis, lelee mmetụta ọgwụgwọ, ma melite nsonaazụ maka enweghị ọmụmụ metụtara ya.

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Ụkpụrụ

Ngwa a na-achọpụta ọgwụ mgbochi endometrial (IgG) n'ime nlele ọbara mmadụ dabere na usoro na-apụtaghị ìhè, yana antigens membran endometrial sachara nke ejiri maka mkpuchi mkpuchi microwells.

Usoro nnwale na-amalite site n'ịgbakwunye ihe nlele serum na olulu mmiri mmeghachi omume antigen precoated maka incubation. Ọ bụrụ na ọgwụ mgbochi endometrial dị na nlele ahụ, ha ga-ejikọta kpọmkwem na antigens endometrial kpuchiri mbụ na microwells, na-eme ogige antigen-antibody kwụsiri ike. Mgbe ewepụchara ihe ndị a na-ekepụtaghị site na ịsa ahụ iji zere nnyonye anya, a na-agbakwunye ọgwụ mgbochi IgG nke akpọrọ horseradish peroxidase.

Na-esote nnabata ọzọ, ọgwụ mgbochi ndị a na-akpọ enzyme na-ejikọta na ogige antigen-antibody dị ugbu a. Mgbe etinyere mkpụrụ osisi TMB, mmeghachi omume agba na-apụta n'okpuru catalysis nke enzyme. N'ikpeazụ, onye na-agụ microplate na-atụle nnabata (A value), nke a na-eji achọpụta ọnụnọ nke anti-endometrial antibodies (IgG) na sample.

Njirimara ngwaahịa

Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	IndirectUsoro
Asambodo	NMPA
Ụdị	Ọbara mmadụ / plasma
Nkọwapụta	48T /96T
Ọnọdụ nchekwa	2-8℃
Ndụ nchekwa	12ọnwa