Ihe mgbochi Cyclic Citrullinated Peptide (CCP) Ngwa ELISA

Ihe mgbochi Cyclic Citrullinated Peptide (CCP) Ihe mgbochi ELISA egosipụtara

Nkọwa dị mkpirikpi:

Ezubere ngwaahịa a maka nchọpụta qualitative in vitro nke anti-cyclic citrullinated peptide antibody n'ime ọbara mmadụ. N'ụlọ ọgwụ, ọ na-adabara dị ka ihe enyemaka diagnostic ngwá ọrụ maka rheumatoid ogbu na nkwonkwo (RA).

Anti-cyclic citrullinated peptide antibodies bụ autoantibodies na-elekwasị anya cyclic citrullinated peptides, ụdị protin antigen gbanwetụrụ. Ọnụnọ ha nwere njikọ chiri anya na ọrịa ogbu na nkwonkwo, ọrịa autoimmune na-adịghị ala ala nke e ji nkwonkwo nkwonkwo na mmebi. E jiri ya tụnyere ihe nrịbama rheumatoid ndị ọzọ, ọgwụ mgbochi ndị a na-egosi nkọwa dị elu maka RA, karịsịa na mmalite nke ọrịa ahụ mgbe mgbaàmà ụlọ ọgwụ na-adịbeghị anya.

Maka ndị ọrịa nwere RA a na-enyo enyo, ịchọpụta ọkwa mgbochi peptide anti-cyclic citrullinated nwere ike inye aka kwado nchoputa ahụ n'oge mmalite, nke dị oke mkpa maka itinye aka n'oge yana igbochi mmebi nkwonkwo na-enweghị ike ịgbanwe. Ọ na-enyekwa aka n'ịmata ọdịiche dị n'etiti RA site na ụdị ọrịa ogbu na nkwonkwo ndị ọzọ nwere mgbaàmà yiri nke ahụ, si otú ahụ na-eduzi ndị dọkịta ka ha mepụta atụmatụ ọgwụgwọ ezubere iche na imeziwanye njikwa ọrịa ahụ.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụkpụrụ

Ngwa a na-achọpụta anti-cyclic citrullinated peptide antibodies (CCP antibodies) n'ime nyocha ọbara mmadụ dabere na usoro na-apụtaghị ìhè, yana antigens peptide cyclic citrullinated dị ọcha ejiri dị ka antigen mkpuchi.

Usoro nnwale ahụ na-amalite site n'ịgbakwunye ihe nlele serum na olulu mmiri mmeghachi omume nke ejirila antigens dị ọcha kpuchie tupu oge eruo, na-esote oge ntinye. N'oge nnabata a, ọ bụrụ na ọgwụ mgbochi CCP dị na nlele ahụ, ha ga-amata kpọmkwem wee jikọta na antigens citrullinated peptide cyclic nke kpuchie na microwells, na-etolite ogige antigen-antibody kwụsiri ike. Iji hụ na izi ezi nke usoro ndị na-esote, a na-ewepụ ihe ndị na-adịghị ejikọta na olulu mmiri mmeghachi omume site na usoro ịsacha, nke na-enyere aka wepụ ihe mgbochi nwere ike site na ihe ndị ọzọ dị na serum.

Na-esote, a na-agbakwunye conjugates enzyme na olulu mmiri mmeghachi omume. Mgbe ntinye nke abụọ gasịrị, conjugates enzyme ndị a ga-ejikọta kpọmkwem na ogige antigen-antibody dị ugbu a, na-eme nnukwu mgbagwoju anya nke na-agụnye antigen, antibody, na enzyme conjugate. Mgbe etinyere ihe ngwọta TMB n'ime sistemu ahụ, enzyme dị na conjugate na-eme ka mmeghachi omume kemịkalụ na mkpụrụ TMB, na-ebute mgbanwe agba a na-ahụ anya. Ike mmeghachi omume agba a na-emetụta kpọmkwem na ọnụọgụ ọgwụ mgbochi CCP dị na nlele mbụ serum. N'ikpeazụ, a na-eji onye na-agụ microplate tụọ nnabata (A uru) nke ngwakọta mmeghachi omume. Site n'ịtụle uru nnabata a, enwere ike ikpebi ọkwa mgbochi CCP dị na nlele ahụ nke ọma, na-enye ihe ndabere a pụrụ ịdabere na ya maka nyocha na nyocha ụlọ ọgwụ dị mkpa.

Njirimara ngwaahịa

Mmetụta dị elu, nkọwapụta na nkwụsi ike

Nkọwapụta ngwaahịa

Ụkpụrụ	Enzyme jikọtara immunosorbent nyocha
Ụdị	IndirectUsoro
Asambodo	NMPA
Ụdị	Ọbara mmadụ / plasma
Nkọwapụta	48T /96T
Ọnọdụ nchekwa	2-8℃
Ndụ nchekwa	12ọnwa